부산대학교 도서관

Pepper (Capsicum annuum L.) is one of the important Solanaceae vegetables. Doubled haploid (DH) derived from anther or microspore culture after spontaneous or artificial chromosome doubling of haploid could be used as the pure parental lines to produce F_1 seeds. The production of the DH has potential to increase the F_1 breeding efficiency as well as to shorten the breeding course of the pepper. However, a successful in vitro DH production is affected by several important factors, including: male gametophyte developmental stage, stress treatment, culture medium and genotype of variety. The effects of low temperature pretreatment in flower buds using 4°C for 24 h or 48 h on embryo induction in anther culture of 10 cultivars of sweet pepper were investigated. Our results showed that 4°C pretreated flower buds had higher embryo induction rates, however, the responses to the duration of low temperature pretreatment were various among cultivars. Among them, the highest 8.3% embryo induction rate was obtained in Known-You 'Royal Star' pretreated at 4°C for 24 h. The effect of different flower bud lengths between 5.00-6.99 mm separated by an interval of 0.5 mm on embryo induction was conducted using Known-You 'Royal Star'. Although a higher embryo induction rate was found by using 5.00-5.99 mm flower bud length, no significant difference among the four flower bud lengths was found.