학술논문
The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone inEscherichia coli
Document Type
Article
Author
Source
Biotechnology Letters; May 1990, Vol. 12 Issue: 5 p335-340, 6p
Subject
Language
ISSN
01415492; 15736776
Abstract
Two types of expression vectors containing thePtacorPLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) inEscherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2terminator was essential for the efficient expression ofpGHin thePtac-driven vectors, but reduced the synthesis of pGH in thePL-vectors. A leaky repression was observed in the JM103 cells containing thePtac-plasmids.