학술논문
Assay for Vitamins D2and D3in the Presence of Tocopherols (Vitamin E) and Vitamin A
Document Type
Article
Author
Source
Journal of Pharmaceutical Sciences; June 1971, Vol. 60 Issue: 6 p913-916, 4p
Subject
Language
ISSN
00223549; 15206017
Abstract
A GLC procedure for determination of vitamins D2and D3is described. By pretreatment consisting of saponification and subsequent esterification of all free hydroxyl groups to their respective propionates, vitamins D2and D3can be isolated from the interference usually encountered from other oil‐soluble vitamins during GLC analysis. The procedure described, including the “internal standard” technique, supplies precise data rapidly, with accuracy comparable to that obtained by the vitamin Dassay method given in USP XVII.