부산대학교 도서관

SummaryThe relative reactivity of a series of nitroaromatic radiosensitizers toward the C(5′′) radical intermediate leading to 8,5′′-cycloadenosine formation in deoxygenated solutions of irradiated polyadenylic acid (poly A) was assessed using standard competition kinetic analysis. Formation of 8,5′′-cycloadenosine was assayed by an indirect, competitive, enzyme-linked immunosorbent assay (ELISA) described in an earlier report. In the absence of oxygen, the nitroaromatics inhibit 8,5′′-cyclonucleoside formation in a way which generally increases with radiosensitizer electron affinity. Although hydroxyl radical scavenging by the nitroaromatics may account for a relatively small decrease in 8,5′′-cyclonucleoside formation, the data suggest that oxidation of the C(5′′) radical intermediate is the more plausible explanation for the decreased yield of the 8,5′′-cyclonucleoside with increasing nitroaromatic electron affinity.