학술논문
scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation
Document Type
Article
Author
Madissoon, E.; Wilbrey-Clark, A.; Miragaia, R. J.; Saeb-Parsy, K.; Mahbubani, K. T.; Georgakopoulos, N.; Harding, P.; Polanski, K.; Huang, N.; Nowicki-Osuch, K.; Fitzgerald, R. C.; Loudon, K. W.; Ferdinand, J. R.; Clatworthy, M. R.; Tsingene, A.; van Dongen, S.; Dabrowska, M.; Patel, M.; Stubbington, M. J. T.; Teichmann, S. A.; Stegle, O.; Meyer, K. B.
Source
Genome Biology; December 2020, Vol. 21 Issue: 1
Subject
Language
ISSN
14747596; 1474760X
Abstract
Background: The Human Cell Atlas is a large international collaborative effort to map all cell types of the human body. Single-cell RNA sequencing can generate high-quality data for the delivery of such an atlas. However, delays between fresh sample collection and processing may lead to poor data and difficulties in experimental design. Results: This study assesses the effect of cold storage on fresh healthy spleen, esophagus, and lung from ≥ 5 donors over 72 h. We collect 240,000 high-quality single-cell transcriptomes with detailed cell type annotations and whole genome sequences of donors, enabling future eQTL studies. Our data provide a valuable resource for the study of these 3 organs and will allow cross-organ comparison of cell types. Conclusions: In conclusion, we present robust protocols for tissue preservation for up to 24 h prior to scRNA-seq analysis. This greatly facilitates the logistics of sample collection for Human Cell Atlas or clinical studies since it increases the time frames for sample processing.