부산대학교 도서관

Mutant strains of Neurospora crassathat lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and β-galactosidase activities are almost identical in tre−strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre−strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The miggene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tregene.