학술논문
Concentrating rotavirus and recombinant enhanced green fluorescent protein fromE. coliusing a temperature-sensitive hydrogel
Document Type
Article
Source
Biotechnology and Bioprocess Engineering; October 1999, Vol. 4 Issue: 3 p224-225, 2p
Subject
Language
ISSN
12268372; 19763816
Abstract
Recombinant enhanced green fluorescent protein (EGFP) fromE. coliwas concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64% and 45%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95% of virus recovery and 93% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins fromE. coli.