부산대학교 도서관

We evaluated platelet activation and adhesion on two plasma polymerized surfaces, Nvinyl pyrrolidone NVP and γbutyro lactone GBL, which have been shown previously to promote endothelial cell growth and adhesion as well as fibronectincoated glass 1 μgcm2 coverslips. Human pulmonary artery endothelial cells were seeded onto coverslips at a low density ∼20,000 cellscm2 and grown to confluence 3–5 days. The materials, both with and without ECs, were then exposed to a shear rate of 400 s−1in a closed loop recirculating flow system containing human plateletrich plasma. Plasma samples were taken at 0, 5, 15, 30, and 60 min and analyzed for platelet and coagulation activation. The coverslips were examined for EC coverage and platelet adherence. EC retention over a 1h period was approximately 75 for all three materials. All three materials without ECs were highly platelet activating having similar Pselectin expression, platelet factor 4 PF4 release, mepacrine uptake, and microparticle production. Both microparticle production and platelet adhesion were significantly lower in ECseeded materials. Dense granule and PF4 release were both slightly diminished in all three materials seeded with ECs. Pselectin expression was reduced slightly for GBL, but remained the same for the other two materials. The ECseeded materials displayed favorable characteristics with respect to platelet activation and adhesion; however, they still demonstrated some thrombogenic tendencies due to EC loss and exposure of the underlying substrate. Therefore, both EC coverage and EC hemostatic function are important factors in determining the thromboresistance of an ECseeded surface. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 57: 419–431, 2001