부산대학교 도서관

Black pepper (Piper nigrumL.) has been commonly utilized in food preparation and traditional medicine in several countries. Seven new amide alkaloids, pipernigramides A–G (3, 10, 38, and 41–44), a new piperic ester, pipernigrester A (48), along with 47 known compounds were isolated from the EtOH extract of P. nigrum. The inhibitory effects on nitric oxide (NO) of all compounds were then evaluated. Among the tested compounds, three of them (42–44) significantly inhibited inducible nitric oxide synthase (iNOS)-mediated NO (IC50= 4.74 ± 0.18, 4.08 ± 0.19, and 3.71 ± 0.32 μM, respectively), and IL-1β, IL-6, TNF-α, and PGE2release in RAW 264.7 cells stimulated by lipopolysaccharide. Moreover, 42–44suppressed IκB degradation and further inhibited the cytosol-nucleus translocation of the p65 subunit by targeting IKK-β. In the carrageenan-induced paw edema test, 42–44demonstrated anti-inflammatory effects as well. These results indicate that all three compounds from P.nigrumhave the potential anti-inflammatory effects.