학술논문
The Effect of Low-dose Irradiation on Unstimulated and PHA-stimulated Human Lymphocyte Subsets
Document Type
Article
Source
International Journal of Radiation Biology; 1988, Vol. 53 Issue: 1 p77-87, 11p
Subject
Language
ISSN
09553002; 13623095
Abstract
A culture system was used to evaluate the radiosensitivity of CD 4+ and CD 8+ T cells, Leu 19+ cells, and B cells obtained from normal adult males. Unstimulated CD 8+ lymphocytes (D0 = 55 cGy) were twice as radiosensitive as CD 4+ cells (D0 = 115 cGy). B cells had an intermediate radiosensitivity (D0 = 100 cGy). Leu 19+ cells were much more radioresistant and expressed a D0 of 550 cGy. When lymphoid cells were irradiated 1 or 4 days before phytohemagglutinin (PHA) stimulation, they were more radiosensitive than if they were first stimulated with PHA and then irradiated. When lymphoid cells were irradiated 1 h after PHA stimulation each lymphocyte subset was characterized by an increase in the D0 to 150 cGy for B cells to 290 cGy for CD4+ cells, and to 240 cGy for CD8+ cells. In contrast, Leu 19+ cells exhibited a decrease in their D0 to 290 cGy after they were stimulated by PHA.