학술논문
The Crystal Structure of the Antibody N10-Staphylococcal Nuclease Complex at 2.9 Å Resolution
Document Type
Article
Author
Source
JMB Online (Journal of Molecular Biology); November 3, 1995, Vol. 253 Issue: 4 p559-575, 17p
Subject
Language
ISSN
00222836; 10898638
Abstract
The three-dimensional structure of the antibody N10 Fab fragment complexed with staphylococcal nuclease (SNase) has been determined to 2.9 Å resolution.Eighteen residues from six-complementarity-determining regions (CDR) recognize an epitope of five distinct SNase segments with a total of 17 residues. The overall shape of the antibody- antigen interface is U-shaped rather than the more or less rectangular seen in other antibody-protein antigen interfaces. Despite the U-shaped interface, the amount of surface buried in the complex, 828 Å2for SNase and 793 Å2for N10, is typical of antibody-protein antigen complexes. Contributing to the shape of the interface is the shortest antibody heavy chain-CDR3 loop reported to date, which probably allows access of bulk solvent in the center of the U interface. Another nusual feature of the N10 antibody is the 15 residue antibody light chain-CDR1, a length seen in only three other reported antibodies. Antibody light chain-CDR1 displays a previously nobserved conformation in its distal portion. Finally, although some of the movement observed in the antibody-bound SNase may be due to crystal contacts, it is clear that some side-chain rearrangements are the result of antigen-antibody interaction.