부산대학교 도서관

We investigated the celite activation of coagulation in heparinized plasma. Celite is shown, using the thrombelastograph, to produce clotting in plasma samples containing pharmacologic levels of heparin (e.g. 1.45 U/ml). We demonstrate that the celite-induced clotting involves Hageman factor, requires calcium, and is not due to celite absorption of heparin. Incubation at 37°C of celite activated plasma samples prior to recalcification, causes a progressive loss in coagulation potential. Unheparinized samples do not exhibit a similar loss. We found that the minimum concentration of heparin required to prevent celite-activated coagulation also reduces celite-activated esterase activity. Massive contact activation is suggested as the most likely explanation for the observed clotting of heparinized samples. Qualitative effects of cellular elements in the plasma are also reported. Finally, the results suggest that using celite activated samples in the thrombelastograph is an improved method for clinical monitoring of heparin effectiveness.