학술논문
A standard calculation methodology for human doubly labeled water studies
Document Type
Article
Author
Speakman, John R.; Yamada, Yosuke; Sagayama, Hiroyuki; Berman, Elena S.F.; Ainslie, Philip N.; Andersen, Lene F.; Anderson, Liam J.; Arab, Lenore; Baddou, Issaad; Bedu-Addo, Kweku; Blaak, Ellen E.; Blanc, Stephane; Bonomi, Alberto G.; Bouten, Carlijn V.C.; Bovet, Pascal; Buchowski, Maciej S.; Butte, Nancy F.; Camps, Stefan G.J.A.; Close, Graeme L.; Cooper, Jamie A.; Creasy, Seth A.; Das, Sai Krupa; Cooper, Richard; Dugas, Lara R.; Ebbeling, Cara B.; Ekelund, Ulf; Entringer, Sonja; Forrester, Terrence; Fudge, Barry W.; Goris, Annelies H.; Gurven, Michael; Hambly, Catherine; El Hamdouchi, Asmaa; Hoos, Marije B.; Hu, Sumei; Joonas, Noorjehan; Joosen, Annemiek M.; Katzmarzyk, Peter; Kempen, Kitty P.; Kimura, Misaka; Kraus, William E.; Kushner, Robert F.; Lambert, Estelle V.; Leonard, William R.; Lessan, Nader; Ludwig, David S.; Martin, Corby K.; Medin, Anine C.; Meijer, Erwin P.; Morehen, James C.; Morton, James P.; Neuhouser, Marian L.; Nicklas, Theresa A.; Ojiambo, Robert M.; Pietiläinen, Kirsi H.; Pitsiladis, Yannis P.; Plange-Rhule, Jacob; Plasqui, Guy; Prentice, Ross L.; Rabinovich, Roberto A.; Racette, Susan B.; Raichlen, David A.; Ravussin, Eric; Reynolds, Rebecca M.; Roberts, Susan B.; Schuit, Albertine J.; Sjödin, Anders M.; Stice, Eric; Urlacher, Samuel S.; Valenti, Giulio; Van Etten, Ludo M.; Van Mil, Edgar A.; Wells, Jonathan C.K.; Wilson, George; Wood, Brian M.; Yanovski, Jack; Yoshida, Tsukasa; Zhang, Xueying; Murphy-Alford, Alexia J.; Loechl, Cornelia U.; Melanson, Edward L.; Luke, Amy H.; Pontzer, Herman; Rood, Jennifer; Schoeller, Dale A.; Westerterp, Klaas R.; Wong, William W.
Source
Cell Reports Medicine; February 2021, Vol. 2 Issue: 2
Subject
Language
ISSN
26663791
Abstract
The doubly labeled water (DLW) method measures total energy expenditure (TEE) in free-living subjects. Several equations are used to convert isotopic data into TEE. Using the International Atomic Energy Agency (IAEA) DLW database (5,756 measurements of adults and children), we show considerable variability is introduced by different equations. The estimated rCO2is sensitive to the dilution space ratio (DSR) of the two isotopes. Based on performance in validation studies, we propose a new equation based on a new estimate of the mean DSR. The DSR is lower at low body masses (<10 kg). Using data for 1,021 babies and infants, we show that the DSR varies non-linearly with body mass between 0 and 10 kg. Using this relationship to predict DSR from weight provides an equation for rCO2over this size range that agrees well with indirect calorimetry (average difference 0.64%; SD = 12.2%). We propose adoption of these equations in future studies.