학술논문
High Titre T Antigen with Minimal Amounts of Structural Antigen in Adenovirus-infected Cells treated with Hydroxyurea
Document Type
Article
Source
Nature; February 1968, Vol. 217 Issue: 5130 p744-745, 2p
Subject
Language
ISSN
00280836; 14764687
Abstract
VIRUS-SPECIFIC non-structural antigens (T antigens) are produced early in the lytic cycle of adenovirus infection and are also found in cells transformed by adenovirus1–3. Synthesis of T antigen, unlike that of the structural antigen, is not dependent on DNA synthesis; for example, fluorodeoxyuridine (FUdR) effectively inhibits the synthesis of viral antigen (V) without markedly suppressing the production of T antigen4,5. Our studies have been designed to produce large quantities of T antigen “free” from viral structural antigen by manipulation of the lytic interaction of adenoviruses in KBcells.