부산대학교 도서관

CD44 was also named CD44 antigen, and the protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the function of CD44 gene effect on triglyceride synthesis in bovine mammary epithelial cells was rarely reported, and its function was not clear.The study detected the effect of CD44 gene on triglyceride synthesis of bovine mammary epithelial cells. After analysis of CD44 mRNA abundance by qRT-PCR and Western blot, overexpression and RNA interference(RNAi) vectors of CD44 gene were constructed and then transfected into bovine mammary epithelial cells(bMECs) to examine the effects of CD44 on milk fat synthesis. The results showed that overexpression of CD44 gene could significantly increase the level of intracellular triglyceride (TG), while CD44 gene knockdown could significantly reduce the TG synthesis in bMECs. This study suggested that the CD44 was important in TG synthesis in bMECs, and the RT2 Profiler PCR array data also suggested that RNAi CD44 lead to genes were upregulated such as acetyl-CoA acyltransferase 1(ACAA1), acyl-CoA thioesterase 8 (ACOT8) etc.（Fold regulation>1.5 or Fold regulation) All of above,the study may suggest the CD44 gene could be a candidate gene to regulate the metabolism of lipid mentalism and involve in fatty acids metabolism.