학술논문
p1220-CTXM, a pKP048-Related IncFIIKPlasmid Carrying BlaCTX-M-14and qnrB4
Document Type
Article
Author
Source
Future Microbiology; September 2017, Vol. 12 Issue: 12 p1035-1043, 9p
Subject
Language
ISSN
17460913; 17460921
Abstract
Aim:This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae1220 carrying blaCTX-M-14and qnrB4. Materials & methods:Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia colithrough conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK. Results:p1220-CTXM was an IncFIIKplasmid genetically closely related to pKP048 and carried resistance markers including blaCTX-M-14, blaDHA-1, qnrB4, sul1and qacEΔ1, all of which were harbored in a 35.7-kb multidrug-resistant region. blaCTX-M-14was located in a truncated ISEcp1-blaCTX-M-14-orf477transposition unit, and qnrB4and blaDHA-1were in a truncated qnrB4-blaDHA-1region. Conclusion:This study provided the insight into the co-occurrence of blaCTX-M-14and qnrB4and the evolution of pKP048-related IncFIIKplasmids.