학술논문
Determination of the catalytic pathway of C 4-leaf pyruvate, orthophosphate dikinase from maize
Document Type
Article
Source
FEBS Letters; January 1990, Vol. 274 Issue: 1 p178-180, 3p
Subject
Language
ISSN
00145793
Abstract
The mechanism of the maize pyruvate, phosphate dikinase-catalyzed conversion of adenosine 5'-triphosphate, orthophosphate and pyruvale to adenosine 5'-monophosphate, inorganic pyrophosphate and phosphoenolpyruvale. respectively, was determined by using transient kinetic techniques. The data obtained demonstrate that catalysis in the maize pyruvate. phosphate dikinasc active site involves initial transfer of the P βP-unit from adenosine 5'- triphosphate to the enzyme to form a pyrophosphorylenzyme intermediate. followed by sequential phosphoryl group transfer to orthophosphate (to form pyrophosphate and a phosphoenzyme intermediate) and pyruvate (to form phosphoenolpyruvate and free enzyme).