부산대학교 도서관

The molecular mechanisms governing calcium signal transduction of corticotropin-releasing factor (CRF) receptors CRF1and CRF2(a)stably expressed in human embryonic kidney (HEK) 293 cells were investigated. Calcium signaling strictly depended on intracellular calcium sources, and this is the first study to establish a prominent contribution of the three major G-protein families to CRF receptor-mediated calcium signaling. Overexpression of Gαq/11and Gα16led to leftward shifts of the agonist concentration-response curves. Blockade of Gαq/11proteins by the small interfering RNA (siRNA) technology partially reduced agonist-mediated calcium responses in CRF1- and CRF2(a)-expressing HEK293 cells, thereby proving a contribution of the Gqprotein family. A small but significant inhibition of calcium signaling was recorded by pharmacological inhibition of Gi/oproteins with pertussis toxin treatment. This effect was mediated by direct binding of Gβγ subunits to phospholipase C. Gi/oinhibition also elevated cAMP responses in CRF receptor-overexpressing HEK293 cells and in Y79 retinoblastoma cells endogenously expressing human CRF1and CRF2(a)receptors, thereby demonstrating natural coupling of Giproteins to both CRF receptors. The strongest reduction of CRF receptor-mediated calcium mobilization was noted when blocking the Gssignaling protein either by cholera toxin or by siRNA. It is noteworthy that simultaneous inhibition of two G-proteins shed light on the additive effects of Gsand Gqon the calcium signaling and, hence, that they act in parallel. On the other hand, Gicoupling required prior Gsactivation.