학술논문
Phosphotyrosine-based Phosphoproteomics for Target Identification and Drug Response Prediction in AML Cell Lines*
Document Type
Article
Author
Source
Molecular and Cellular Proteomics (MCP Online); May 2020, Vol. 19 Issue: 5 p884-899, 16p
Subject
Language
ISSN
15359476; 15359484
Abstract
Phosphotyrosine (pY)-specific phosphoproteome profiles have been determined for 16 AML cell lines through a pY immunoprecipitation-based protocol. Kinase activity inference using dedicated ranking analysis, combining kinome-, activation loop-, and substrate-based analyses, identified potential drivers for all cell lines and driver function was confirmed through drug experiments. Results for two patient samples show potential of phosphoproteomics in a clinical setting.