학술논문
A novel synthetic-genetic-array–based yeast one-hybrid system for high discovery rate and short processing time
Document Type
Article
Author
Yeh, Chung-Shu; Wang, Zhifeng; Miao, Fang; Ma, Hongyan; Kao, Chung-Ting; Hsu, Tzu-Shu; Yu, Jhong-He; Hung, Er-Tsi; Lin, Chia-Chang; Kuan, Chen-Yu; Tsai, Ni-Chiao; Zhou, Chenguang; Qu, Guan-Zheng; Jiang, Jing; Liu, Guifeng; Wang, Jack P.; Li, Wei; Chiang, Vincent L.; Chang, Tien-Hsien; Lin, Ying-Chung Jimmy
Source
Genome Research; 2019, Vol. 29 Issue: 8 p1343-1351, 9p
Subject
Language
ISSN
10889051; 15495469
Abstract
Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cistargets. Yeast one-hybrid (Y1H) is one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis. The system has a transcription factor–DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. The system also offers the highest accuracy in identifying TF–DNA interactions that can be authenticated in vivo by chromatin immunoprecipitation. With these unique features, this meiosis-directed Y1H system is particularly suited for constructing novel and comprehensive genome-scale gene regulatory networks for various organisms.