학술논문
Transcription of Yeast COX6, the Gene for Cytochrome cOxidase Subunit VI, Is Dependent On Heme and on the HAP2 Gene
Document Type
Article
Author
Source
Journal of Biological Chemistry; April 1989, Vol. 264 Issue: 12 p7005-7008, 4p
Subject
Language
ISSN
00219258; 1083351X
Abstract
The COX6gene encodes subunit VI of cytochrome c oxidase. Previously, this gene and its mRNAs were characterized, and its expression has been shown to be subject to glucose repression/derepression. In this study we have examined the effects of heme and the HAP1(CYP1) and HAP2genes on the expression of COX6. By quantitating COX6RNA levels and assaying β-galactosidase activity in yeast cells carrying COX6-lacZfusion genes, we have found that COX6is regulated positively by heme and HAP2, but is unaffected by HAP1. Through 5′ deletion analysis we have also found that the effects of heme and HAP2on COX6are mediated by sequences between 135 and 590 base pairs upstream of its initiation codon. These findings identify COX6 as the fourth respiratory protein gene that is known to be regulated positively by heme and HAP2. The other three, CYC1, COX4, and COX5a, encode iso-1-cytochrome c, cytochrome coxidase subunit IV, and an isolog, Va, of cytochrome coxidase subunit V, respectively. Thus, it appears that the biogenesis of two interacting proteins, cytochrome cand cytochrome coxidase, in the mitochondrial respiratory chain, are under the control of common factors.