학술논문

A practical approach to genome assembly and annotation of Basidiomycota using the example of Armillaria
Document Type
Article
Source
Biotechniques; 20230101, Issue: Preprints
Subject
Language
ISSN
07366205
Abstract
Technological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillariaspecies. Most Armillariaspecies are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillariagenome projects are evaluated in this review. Infographic guidelines and a database of resources to facilitate future Armillariagenome projects were developed. Knowledge gained from genomic studies of Armillariaspecies is summarized and prospects for further research are provided. This guide can be applied to other diploid and dikaryotic fungal genomics.