학술논문
Mycobacterium paratuberculosis antigen D: characterization and evidence that it is a bacterioferritin
Document Type
Article
Source
Journal of Clinical Microbiology; August 1991, Vol. 29 Issue: 8 p1652-1658, 7p
Subject
Language
ISSN
00951137; 1098660X
Abstract
By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.