학술논문
Creatine supplementation improves intracellular Ca2+handling and survival in mdxskeletal muscle cells
Document Type
Article
Source
FEBS Letters; November 1998, Vol. 439 Issue: 3 p357-362, 6p
Subject
Language
ISSN
00145793
Abstract
Dystrophic skeletal muscle cells from Duchenne muscular dystrophy (DMD) patients and mdxmice exhibit elevated cytosolic Ca2+concentrations ([Ca2+]c). Pretreatment of mdxmyotubes for 6–12 days with creatine (20 mM) decreased the elevation in [Ca2+]cinduced by either high extracellular Ca2+concentrations or hypo‐osmotic stress to control levels. 45Ca2+influx measurements suggest that creatine lowered [Ca2+]cby stimulating sarcoplasmic reticulum Ca2+‐ATPase. Creatine pretreatment increased levels of phosphocreatine but not ATP. Furthermore, myotube formation and survival were significantly enhanced by creatine pretreatment. Therefore, creatine supplementation may be useful for treatment of DMD.