학술논문
HPLC determination of catabolic amines in silage using their dansyl derivatives and an electrochemical detector
Document Type
Article
Author
Source
Chromatographia; March 1989, Vol. 27 Issue: 5-6 p216-220, 5p
Subject
Language
ISSN
00095893; 16121112
Abstract
The use of the electrochemical detector (EC) in the high performance liquid chromatography (HPLC) of dansyl derivatives of biogenic amines is reported. Isocratic and gradient elution patterns of synthetic mixtures of putrescine, cadaverine, 1,6-diaminohexane, tryptamine, histamine, tyramine, spermine and spermidine on a reversed phase column are shown. Hydrodynamic voltammograms of standard compounds are presented. The detection limits of the EC are compared with those of ultraviolet and spectrofluorimetric detectors. A chromatographic profile of amines from the proteolytic catabolism of maize silage byClostridia is shown.