학술논문
Functions of a GyrBA Fusion Protein and Its Interaction with QnrB and Quinolones
Document Type
Article
Source
Antimicrobial Agents and Chemotherapy; August 2015, Vol. 59 Issue: 11 p7124-7127, 4p
Subject
Language
ISSN
00664804; 10986596
Abstract
ABSTRACTIn order to study the interactions between Escherichia coliDNA gyrase and the gyrase interacting protein QnrB in vivo, we constructed a gyrB-gyrAfusion and validated its ability to correct the temperature-sensitive growth of gyrAand gyrBmutants. Like wild-type gyrA, the gyrB-gyrAfusion complemented a quinolone-resistant gyrAmutant to increase susceptibility. It functioned as an active type II topoisomerase, catalyzed negative supercoiling of DNA, was inhibited by quinolone, and was protected by QnrB.