부산대학교 도서관

A medium containing trypan blue, gentamicin, and chloramphenicol is introduced for the detection of Cryptococcus neoformansand Cryptococcusspecies from clinical samples. Ten recently isolated strains of Cryptococcusspecies as well as several clinical isolates of C. neoformansincorporated trypan blue and produced dark blue colonies on this mycological medium, whereas other common yeasts were light blue. The laboratory diagnosis of two cases of cryptococcosis was accomplished by the isolation of C. neoformanson the antibiotic-dye-containing medium. Compared to conventional media supporting large numbers of Pseudomonas aeruginosaand other gram-negative bacilli, the new medium was selective for yeasts. In one instance, the colonization of the respiratory tract by C. neoformanswhich led to fungemia was traced by the use of the antibiotic-dye medium. The antibiotic mixture, utilized herein, was more effective in suppressing bacteria contained in samples from patients than a medium containing cycloheximide and chloramphenicol.