부산대학교 도서관

The aim of research work was to prepare and optimize a bacterial cellulose–carbopol hydrogel loaded with AILE (Azadirachta indicaextract) for burn wound healing. The preparation process of hydrogel was optimized by using response surface methodology in which concentration of carbopol, bacterial cellulose, and crosslinking agent were varied and various responses were recorded that included swelling %, entrapment efficacy, and water retention capacity. The morphological structure and physical properties were characterized by SEM, FTIR, rheological properties, XRD, swelling %, water retention capacity, zeta potential, and stability study. The effect of pH on release mechanism of AILE was elucidated by fitting in vitro release data to a series of mathematical models, i.e., zero-order, first-order, Higuchi, and Hixson–Crowell. The prepared hydrogel was tested for anti-microbial activity against both gram-positive and gram-negative bacteria. In vitro anti-inflammatory activity was evaluated by using hemolysis inhibition assay. The results revealed that AILE and hydrogels possessed antimicrobial, and anti-inflammatory properties. Thus, AILE loaded novel hydrogels have great potential for burn wound management. In the current research work, the hydrogel was prepared by using blending bacterial cellulose with carbopol and crosslinked by N, N-methylene bisacrylamide with optimized concentration of 3.96% bacterial cellulose, 0.52% carbopol, and 0.5% crosslinking agent.