학술논문
Detection and Measurement of Nucleotide Excision Repair Synthesis by Mammalian Cell Extracts in Vitro
Document Type
Article
Source
Methods: A Companion to Methods in Enzymology; April 1995, Vol. 7 Issue: 2 p163-175, 13p
Subject
Language
ISSN
10462023; 10959130
Abstract
Nucleotide excision repair of damaged DNA can be carried out in a cell-free environment, making the process amenable to biochemical study and manipulation. In this article, methods for a versatile repair synthesis assay are described. Protocols are given for preparing plasmid DNA substrates, preparing whole cell extracts, performing repair synthesis reactions, and analyzing the data obtained. Methods are also described for in vitro complementation of repair synthesis defects and for carrying out two-stage incision/synthesis reactions.Copyright 1995, 1999 Academic Press