학술논문
Pacific Rim Forestry / Silviculture and Forest Ecology
Document Type
Article
Author
Negi, Vishal Singh; Pal, Archana; Singh, Ratnesh; Borthakur, Dulal; Pala, Archana; Negi, Vishal Singh; Borthakur, Dulal; Rushanaedy, Isabel; Jones, Tyler; Dudley, Nicklos; Borthakur, Dulal; Yafuso, Jannai; Negi, Vishal Singh; Borthakur, Dulal; Friday, Kathleen; Sanney, Jacob; Nithan, Maxson; Jonathan, Rickson; Waguk, Erick; Friday, Kathleen; Michael, Uelbil Puanani; Sadang, Omekrael; Friday, Kathleen; Eperiam, Eugene; Marquez, Wendolin Roseo; Friday, Kathleen; Maugalei, Fa'afa'i Tony; Sue, John Ah; Friday, Kathleen; Jones, Tyler; Dudley, Nicklos; Strom, Brian; Smith, Sheri; Fischer, Joseph; Jones, Tyler; Dudley, Nicklos; Borthakur, Dulal; Yeh, Aileen; Sniezko, Richard; Cannon, Philip; James, Robert; Schenck, Susan; Raboin, Erin; Johnson, M. Tracy; Denslow, Julie; Liu, Zhanfeng; Gurr, Neil; Schmaedick, Mark; Whistler, Arthur; Fischer, Lisa; Jones, Tyler; Dudley, Nicklos; James, Robert; Borthakur, Dulal; Cannon, Philip; Sniezko, Richard; Schenck, Susan; Kolka, Randy; Hanson, Paul; Norby, Rich; Palik, Brian; Wullschleger, Stan; Garten, Charles; Sebestyen, Steve; Thornton, Peter; Bradford, John; Mulhollend, Pat; Todd, Don; Iversen, Colleen; Warren, Jeff; Peterson, Kristin; Shiraishi, Ayami; Uchida, Janice; Tacconelli, Michelle; Loewenstein, Edward; Bell, Conor; Kettler, Kenneth; Premer, Michael; Oikarinen, Kara; Dunnack, Eric; Pickens, James; Burkowski, Marilynn; Gazal, Rico; Dugan, Sean; Oswald, Brian; Balice, Randy; Unger, Daniel; Kreye, Melissa; Stein, Taylor; Ober, Holly; Kobziar, Leda; Bohn, Kimberly; Carter, Douglas; Peterson, Nancy; Doruska, Paul; Vacchiano, Giorgio; Berretti, Roberta; Motta, Renzo; Michaels, Christy; Oswald, Brian; Williams, Hans; Farrish, Kenneth; Schuler, Jamie; Litton, Creighton; Giardina, Christian; Crow, Susan; Fortini, Lucas; Schultz, Emma; Seymour, Robert; Weiskittel, Aaron; Droske, Christine; Morgan, Penelope; Davis, Anthony S.; Robichaud, Peter R.; Lewis, Sarah; Hudak, Andrew T.; Lentile, Leigh B.; Francis, Paul; Orr, Blair; Long, Michael; Litton, Creighton; Giardina, Christian; Sparks, Jed; Macy, Thomas; Goebel, Charles; Hix, David; Miller, David; Rudnicki, Mark; Hiscox, April; Su, Hong Bing; Kolb, Thomas; Previant, Wilfred; Nagel, Linda; Webster, Christopher; Fuller, Angela; Harrison, Daniel; DeRose, Justin; Shaw, John; Long, Jim; Bentz, Barbara; Holzmueller, Eric; Gibson, David; Suchecki, Paul; Stuhlinger, Chris; Silver, Emily; D'Amato, Anthony; Fraver, Shawn; Palik, Brian; Strommer, Shane; Teets, Andrew; Houghton, Craig; Gundlach, Andrew; Fritz, Brian; Brantley, Elizabeth; Stuhlinger, Chris; Cunningham, Kyle; Pelkki, Matthew; Caldwell, Benjamin; Kusbach, Antonin; Shaw, John; Long, Jim; Eiche, Nik; Strom, Brian; Smith, Sheri; Jones, Dryw; Lightle, Nicole; Heckathorn, Scott; Morton, Nathaniel; Weigle, Brad; McGown, Kathryn; Miller, Joshua; Stewart, Jane; Ross-Davis, Amy; Davis, Anthony S.; Chhin, Sophan; Mbow, Cheikh; Sambou, Bienvenu; Skole, David; Gottschalk, Kurt; Miller, Gary; Brose, Patrick; Tolley, Grant; Battaglia, Mike; Jain, Theresa; Han, Han-Sup; Graham, Russell; Keyes, Christopher; Fried, Jeremy
Source
Journal of Forestry; December 2011, Vol. 109 Issue: 8 p557-570, 14p
Subject
Language
ISSN
00221201; 19383746
Abstract
Leucaena leucocephala(leucaena) is an important agroforestry tree of tropics and subtropics due to its protein rich foliages, ability to grow on poor soils, and high level of tolerance to various stresses including drought and diseases. We hypothesized that leucaena have a unique set of genes which confer tolerance to various abiotic and biotic stresses and these genes are either absent or not expressed in other legumes that are susceptible to these stress conditions. Interspecies suppression subtractive hybridization (iSSH) was performed to identify some unique genes from leucaena using cDNAs from leucaena and Acacia confusa (acacia) as the tester and driver, respectively. We identified 100 leucaena genes showing homology to various plant genes with known functions, of which 23 genes had homology to various stress-related proteins including chitinase, PR-10 protein, wound-stress protein, type-2 metallothionein, thaumatin-like protein, disease resistance response protein, cysteine proteinase, and kunitz trypsin inhibitor. We also identified 50 leucaena genes which either had homology to hypothetical proteins (HyPs) or had no homology at all. Putative functions of 15 HyPs were predicted using conserved domain and PSI-BLAST analyses. Linear motif analyses identified 17 HyPs with MAPK recognition motif, which is involved in signaling pathways, including stress responses. This study identified 23 known and some putative unique stress-related genes from leucaena which may serve as a useful source of stress-tolerance genes for tree and crop improvement in the future.