학술논문
Exploring Regulation of Protein O-Glycosylation in Isogenic Human HEK293 Cells by Differential O-Glycoproteomics*[S]
Document Type
Article
Author
Source
Molecular and Cellular Proteomics (MCP Online); July 2019, Vol. 18 Issue: 7 p1396-1409, 14p
Subject
Language
ISSN
15359476; 15359484
Abstract
The hereto most comprehensive characterization of nonredundant contributions of individual GalNAc-T isoforms to the O-glycoproteome of the human HEK293 cell using quantitative differential O-glycoproteomics on a panel of isogenic HEK293 cells with knockout of GalNAc-T genes (GALNT1, T2, T3, T7, T10, or T11). The engineered HEK293 cell panels are useful for expression and analysis of the regulation of O-glycosylation and enables wider studies of the biological functions of GalNAc-T isoform-specific glycosylation.