학술논문
Characterization of the spoTgene of Escherichia coli
Document Type
Article
Author
Source
Journal of Biological Chemistry; September 1989, Vol. 264 Issue: 25 p15074-15082, 9p
Subject
Language
ISSN
00219258; 1083351X
Abstract
The Escherichia coli spoTgene encodes a guanosine-3′,5′-bispyrophosphate (ppGpp) 3′-pyrophosphohydrolase known to be responsible for cellular (ppGpp) degradation. The DNA sequence of the spoTregion is presented. The spoTgene is deduced to be 702 codons long, with a probable UUG initiation codon, and a deduced mass of 79,342 daltons. Two spoTmutations (spoT202and spoT203) have been localized to an open reading frame by complementation of function as well as by genetic marker rescue. The ability to overexpress the spoTgene is limited, but enough ppGppase activity can be made to reverse ppGpp accumulation during the stringent response to amino acid starvation.