학술논문
Isolation of Pleurotus floridaderived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice
Document Type
Article
Author
Source
Food Science and Human Wellness; July 2021, Vol. 10 Issue: 4 p490-496, 7p
Subject
Language
ISSN
22134530
Abstract
Our earlier work showed that hydromethanol extract (HME) of Pleurotus floridahad antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitroanti-acetylcholinesterase (Ellman method) and antioxidant (DPPH scavenging assay). The most active constituent was evaluated in vivoemploying i.c.v. streptozotocin (STZ) induced dementia in mice. Morris water maze test was used for evaluating memory, followed by biochemical estimations and histopathological studies. Bioactivity guided fractionation resulted in isolation of resveratrol (identified by IR, NMR and MS) and its content in P. floridafruiting bodies was 0.0098% (m/m, by a validated TLC densitometric method). It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress. The observed effects might be the presence of resveratrol. Our results further endorse that P. floridaderived resveratrol can be a promising therapy for Alzheimer's disease.