학술논문
Monoclonal antibodies to human urokinase identify the single-chain pro-urokinase precursor.
Document Type
Article
Author
Source
Proceedings of the National Academy of Sciences of the United States of America; January 1984, Vol. 81 Issue: 1 p110-114, 5p
Subject
Language
ISSN
00278424; 10916490
Abstract
Monoclonal antibodies have been obtained that recognize either the A or B chain of human urinary urokinase. These antibodies identify human urokinase-producing cells and the product of urokinase mRNA. Anti-urokinase monoclonal antibodies precipitate an approximately equal to 54,000-dalton protein synthesized in vitro in a rabbit reticulocyte cell-free system. This pro-urokinase appears to be the precursor of both A and B chains of human urinary urokinase. Urokinase mRNA in human kidney constitutes only 0.1% or less of total poly(A)+ RNA.