학술논문
Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence properties.
Document Type
Article
Author
Source
Infection and Immunity; October 2000, Vol. 68 Issue: 10 p6082-6, 5p
Subject
Language
ISSN
00199567; 10985522
Abstract
We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.