학술논문
Fluorescent Hybridization of Mycobacterium lepraein Skin Samples Collected in Burkina Faso
Document Type
Article
Author
Source
Journal of Clinical Microbiology; April 2020, Vol. 58 Issue: 5
Subject
Language
ISSN
00951137; 1098660X
Abstract
Leprosy is caused by Mycobacterium leprae, and it remains underdiagnosed in Burkina Faso. We investigated the use of fluorescent in situhybridization (FISH) for detecting M. lepraein 27 skin samples (skin biopsy samples, slit skin samples, and skin lesion swabs) collected from 21 patients from Burkina Faso and three from Côte d’Ivoire who were suspected of having cutaneous leprosy. In all seven Ziehl-Neelsen-positive skin samples (four skin biopsy samples and three skin swabs collected from the same patient), FISH specifically identified M. leprae, including one FISH-positive skin biopsy sample that remained negative after testing with PCR targeting the rpoBgene and with the GenoType LepraeDR assay.