학술논문
Development of an Enzyme-Mediated Assay for Phenylalanine in Blood Spots
Document Type
Article
Source
Annals of Clinical Biochemistry; March 1994, Vol. 31 Issue: 2 p140-146, 7p
Subject
Language
ISSN
00045632
Abstract
We describe the optimization of a rapid procedure for the elution of phenylalanine from blood spots and the estimation of the amino acid eluted using an enzyme-mediated assay linked to a colorimetric detection system. The method is rapid, fully quantitative, interference-free, accurate and precise unlike many of the methods currently employed for phenylalanine determination. The performance of the proposed method may necessitate the review of reference ranges and cut-off assignment strategies.