부산대학교 도서관

Noninvasive imaging of endogenous retinal fluorophores, including vitamin A derivatives, is vital to developing new treatments for retinal diseases. Here, we present a protocol for obtaining in vivotwo-photon excited fluorescence images of the fundus in the human eye. We describe steps for laser characterization system alignment, positioning human subjects and data registration. We detail data processing and demonstrate analysis with example datasets. This technique allays safety concerns by allowing for the acquisition of informative images at low laser exposure.