학술논문
Effects of chloroquine and cytochalasin B on the infection of cells by Sindbis virus and vesicular stomatitis virus
Document Type
Article
Author
Source
The Journal of Virology; March 1981, Vol. 37 Issue: 3 p1060-1065, 6p
Subject
Language
ISSN
0022538X; 10985514
Abstract
The effects of cytochalasin B and chloroquine on the process of endocytosis of Sindbis virus particles and polystyrene spheres were determined by electron microscopy. The effects of these agents on the process of infection (attachment, penetration, and uncoating) of BHK-21 cells by Sindbis virus and vesicular stomatitis virus were also determined. Cytochalasin B completely blocked ingestion of Sindbis virus particles or latex spheres by BHK cells but had no effect on the ability of Sindbis virus or vesicular stomatitis virus to infect or replicate in BHK cells. Chloroquine did not inhibit the ingestion of either latex spheres or virus particles but greatly reduced the yields of virus produced. These data suggest that endocytosis is not essential for the infection of cultured cells by Sindbis virus or vesicular stomatitis virus.