학술논문
Preclinical Testing of CAR T Cells in a Patient-Derived Xenograft Model of Glioblastoma
Document Type
Article
Author
Source
STAR Protocols; December 2020, Vol. 1 Issue: 3
Subject
Language
ISSN
26661667
Abstract
Glioblastoma (GBM) is the most common malignant adult brain tumor that is resistant to the standard care therapy. Advances in chimeric antigen receptor (CAR) T cell therapies have spurred renewed interest in developing CAR T cell therapies to target chemoradiotherapy-resistant brain tumor-initiating cells. This protocol shows how to isolate peripheral blood mononuclear cells from healthy donors and generate CAR T cells for the antigens of interest, and how to intracranially inject the CAR T cells into a patient-derived xenograft mouse model of GBM.