학술논문
Overexpression of Plant Uncoupling Mitochondrial Protein in Transgenic Tobacco Increases Tolerance to Oxidative Stress
Document Type
Article
Source
Journal of Bioenergetics and Biomembranes; June 2003, Vol. 35 Issue: 3 p203-209, 7p
Subject
Language
ISSN
0145479X; 15736881
Abstract
An Arabidopsis thalianacDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northernblot, revealed variable levels of transgene expression. Antibody probing ofWestern blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significantincrease in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide thefirst biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.