학술논문
Detection and isolation of Helicobacter mustelae from stoats in New Zealand
Document Type
Article
Author
Source
New Zealand Veterinary Journal; June 2003, Vol. 51 Issue: 3 p142-145, 4p
Subject
Language
ISSN
00480169; 11760710
Abstract
AIM: To determine the incidence of Helicobacter mustelae in stoats (Mustela erminea) in New Zealand.METHODS: Helicobacter-like organisms and total genomic DNA were isolated from gastric tissue of stoats and identified using a combination of bacterial culture, phenotypic testing and molecular techniques.RESULTS: A Helicobacter-specific 16S rRNA polymerase chain reaction product was detected in 16/32 gastric tissue biopsies tested. Nine of 13 partially sequenced 16S rRNA DNA identified H. mustelae 16S DNA. Bacteria, subsequently identified as H. mustelae, were successfully cultured from the stomachs of 4/32 stoats. Other Helicobacter species were also identified by DNA sequence analysis, but were not cultured.CONCLUSIONS: Helicobacter mustelae is present in stoats from both the North and South Islands of New Zealand.