부산대학교 도서관

Hepatocyte transplantation is an attractive alternative in the treatment of congenital errors of metabolism. Liver cells grafting offers the ability of immunomodulate and cryopreserve the hepatocytcs. The aim of this work was to evaluate the efficacy of singeneic liver cell transplantation in a rat mutant unable to synthetize ascorbic acid (AsA) in hte liver (CDS od/od). A total of 4 non-deficient rats (ODS +/+) were used as donors. Hepatocyte isolation was carried out according collagenase perfusion. The proportion of cells excluding trypan blue (0.2%) was counted; viability ranging from 90% to 93%. Hepatocyte transplantation was performed by injection into the splenic pulp of recipient animals of 0.5 - 1.0 ml of cell suspension containing 90 million viable hepatocytes. The recipient animals (ODS od/od rats) were divided into two groups. Group I (n=8) which was transplanted into the spleen; Group II (n =4) which was not transplanted; a control group of ODS */+ rats (Group III; n=5) was assessed. The deficient animals were fed a standard rat diet and water containing 3g/1 of AsA (Fuso, Osaka, Japan) during 4 weeks before transplantation and grafted 1 week later. The survival in the transplanted group (Group 1) was 63% at 5 postoperative week and 50% in the non-transplanted group (Group II). Hematoxylin-eosin staining revealed the existence of hepatocytes in spleens of transplanted animals. In spite of the improvement in the survival, all animals developed signs of AsA deficiency.This study confirms previous works for considering that an enzyme deficiency could be corrected by cellular transplant. Further studies are necessary to prove the usefulness of liver cell transplantation and the timing of grafting.