학술논문
Fast Identification and Quantification of Uropathogenic E. colithrough Cluster Analysis
Document Type
Article
Author
Vendeville, Jean-Baptiste; Kyriakides, Mathew John; Takebayashi, Yuiko; Rama, Sylvain; Preece, James; Samphire, Jenny; Ramos-Soriano, Javier; Amieva, Abraham Mauleon; Holbrow-Wilshaw, Maisie Emma; Gordon Newman, Henry Ralph; Kou, Sio Lou; Medina-Villar, Sandra; Dorh, Neciah; Dorh, Josephine Ndoa; Spencer, James; Galan, M. Carmen
Source
ACS Biomaterials Science & Engineering; January 2022, Vol. 8 Issue: 1 p242-252, 11p
Subject
Language
ISSN
23739878
Abstract
Rapid diagnostic tools to detect, identify, and enumerate bacteria are key to maintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broad-spectrum agents. In this study, a 15 min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC 12241), and uropathogenic Escherichia colistrains (NCTC 9001, NCTC 13958, J96, and CFT073) at clinically relevant concentrations in tryptic soy broth (103–108CFU/mL) and in urine (105–108CFU/mL). This fast, simple, and efficient assay offers a step forward toward efficient point-of-care diagnostics for common urinary tract infections.