학술논문
Optogenetic and chemogenetic manipulation of seizure threshold in mice
Document Type
Article
Source
STAR Protocols; 20220101, Issue: Preprints
Subject
Language
ISSN
26661667
Abstract
Here, we present a protocol using optogenetics or chemogenetics to assess the neuronal circuits contributing to seizure initiation. Both approaches allow for targeted control of neuronal populations in vivoand can be combined with experimental manipulations to acutely induce seizures in rodent models. We describe how to (1) introduce and (2) activate optogenetic or chemogenetic actuators, while (3) inducing seizures via hyperthermia in a mouse model of epilepsy. This protocol can be adapted for use in other induced seizure models.