학술논문
Effects of tumour necrosis factor α on bone marrow aspirates of patients with acute myelogenous leukemia determined by flow-cytometric cell-cycle analysis
Document Type
Article
Author
Source
Journal of Cancer Research and Clinical Oncology; January 1992, Vol. 118 Issue: 1 p56-60, 5p
Subject
Language
ISSN
01715216; 14321335
Abstract
Summary Tumour necrosis factor a (TNFa) exerts cytotoxic and antiproliferative effects on neoplastic cells. It has been used as a therapeutic agent for solid tumours and haematological malignancies. We report on the ex vivo determination of the effect of recombinant human rhuTNFa on bone marrow aspirates by a bromodeoxyuridine/propidium iodide method. Cell samples were drawn after 0.5, 2, 4, 6, 8, 10, 22, and 25 h from shortterm suspension bone marrow cultures from patients with acute myelogenous leukemia (AML). Flow-cytometric cell-cycle analysis was performed after double DNA staining with propidium iodide and anti-BrdU antibodies. By this method the effect of rhuTNFa on cell proliferation can be evaluated after only 35 h. In about two-thirds of the bone marrow aspirates of AML an inhibiting effect on rhuTNFa can be demonstrated, developing to its full extent after 10 h.