학술논문
Sequence and comparative analysis of three Enterobacter cloacae ampC β-lactamase genes and their products
Document Type
Article
Author
Source
Biochemical Journal; March 1988, Vol. 250 Issue: 3 p753-760, 8p
Subject
Language
ISSN
02646021; 14708728
Abstract
The sequences of three Enterobacter cloacae ampC beta-lactamase genes have been determined. The deduced amino acid sequences are very similar: out of a total of 361 residues, only eight positions were found to be variable, and several mutations yielded residues with very similar properties. The kinetic properties of two of the enzymes were not significantly different. The three enzymes also exhibited a high degree of homology (greater than 70%) with the ampC beta-lactamases of Escherichia coli K12 and Citrobacter freundii, confirming the homogeneity of class-C beta-lactamases.