학술논문
The active sites of the β-lactamases of Streptomyces cacaoi and Streptomyces albus G.
Document Type
Article
Author
Source
Biochemical Journal; June 1987, Vol. 244 Issue: 2 p427-432, 6p
Subject
Language
ISSN
02646021; 14708728
Abstract
The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especially homologous, and none of them exhibited a high degree of homology with the Streptomyces R61 DD-peptidase. Our data confirm that, as a family of homologous enzymes, class A is rather heterogeneous, with only a small number of conserved residues in all members of the class.