부산대학교 도서관

The noncallusing morphogenetic properties of Citrusjuice vesicles cultured in vitro are unknown and were herein studied. Juice vesicles isolated from 120–180‐day‐old fruits are capable of proliferation via adventitious vesicle branching in vitro. Gibberellic acid levels of 1–100 mg/L greatly enhanced adventitious vesicle branching while delaying vesicle senescence as evidenced by the vesicles' ability to retain its green color for up to 4 to 6 mo in culture. Vesicles grown on media without gibberellic acid readily matured, turning opaque within 2 mo in culture and rarely produced adventitious branches. Additions of 0.1 mg/L α‐naphthaleneacetic acid or 1.0 mg/L benzylaminopurine to media containing 10 mg/L gibberellic acid further enhanced vesicle branching in some species and cultivars. Adventitious juice vesicles have been induced from a variety of citrus species, including Citrus grandis(L.) Osb., C. hystrixDC., C. limon(L.) Burm. f., C. medicaL., C. paradisiMacf., C. reticulataBlanco, and C. sinensis(L.) Osb. Adventitious vesicle branches originate from primordia initiated on the surface of the cultured vesicle. These primordia commonly occurred on the terminal meristem region of the vesicle. However, some species (e.g., C. grandisand C. paradisi) produced adventitious vesicle branches from their bodies and stalks as well. Distinct vesicle branches begin to appear on preformed vesicles after 30–60 days in culture. A survey to determine the natural occurrence of vesicle branching in various Citrusspecies was also conducted. Natural vesicle branching commonly occurred in C. grandis, C. paradisi, and in some cultivars of C. reticulata, but was absent in most citrus species and cultivars (e.g., C. aurantifolia(Christm.) Swing., C. canaliculataHort. ex Y. Tan., C. hystrix, C. limon, C. medica, C. sinensis). It was found that some species which failed to exhibit the adventitious branching phenomenon in nature did so in vitro (e.g., C. hystrix, C. medica, and C. sinensis).