학술논문

Identification of Streptococcus agalactiaeIsolates from Various Phylogenetic Lineages by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Document Type
Article
Source
Journal of Clinical Microbiology; July 2009, Vol. 47 Issue: 7 p2284-2287, 4p
Subject
Language
ISSN
00951137; 1098660X
Abstract
ABSTRACTVariations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiaeisolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative “species-identifying” biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.